别称 | wt-Streptavidin, wt-SA |
表达宿主 | E.coli |
纯度 | >90% as judged by SDS-PAGE analysis |
分子量 | 66 kDa |
比活性 | >14 U/mg |
产品性状 | Liquid, colorless and clear |
缓冲液 | PBS pH7.4 |
应用 | Conjugated enzymes, fluorescein, colloidal gold and other active substances can also be coupled to solid-phase carriers such as magnetic beads, microspheres, and microplates. |
稳定性 & 储存条件 |
For long term use, aliquot and store at -80°C after reconstitution (avoiding frequent freeze-thaws). |
SDS-PAGE |
Streptavidin (SA) is a tetramer protein secreted by Streptomyces during cultivation, with a molecular weight of approximately 66kDa. Streptomycin has similar biological characteristics to avidin, with each subunit possessing a site capable of tightly binding to biotin. It can form stable non covalent specific complexes with the imidazole copper ring of biotin. Streptomycin is often coupled with other active substances such as enzymes, fluorescein, colloidal gold, etc. It can also be coupled with solid-phase carriers such as magnetic beads, microspheres, and microplates to participate in signal amplification in immunology. It is widely used in ELISA, IHC, immunochromatography, protein purification, luminescence diagnosis, biosensors, and the production of biochips.
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